Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: Expression of RAC1 mRNA and its protein levels. (A,B) Expression of RAC1 mRNA in tumor tissues and adjacent tissues. (C) Expression of RAC1 mRNA in different stages of tumors. (D,E) Immunohistochemical analysis of RAC1 expression in tumor tissues and adjacent tissues. (F) Comparison of differences in RAC1 expression between tumor tissues and adjacent tissues. *, P<0.05; **, P<0.01; ****, P<0.0001. ns, not significant; LIHC, liver hepatocellular carcinoma; RAC1 , Ras-related C3 botulinum toxin substrate 1; TPM, transcripts per million.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques: Expressing, Immunohistochemical staining, Comparison

Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: Relationship between RAC1 expression level and prognosis. (A) OS; (B) DSS; (C) DFI; (D) PFI. DFI, disease-free interval; DSS, disease-specific survival; OS, overall survival; PFI, progression-free interval; RAC1 , Ras-related C3 botulinum toxin substrate 1.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: Analysis of prognostic factors and establishment of a prognostic prediction model for patients with HCC. (A) Univariate Cox analysis. (B) Multivariate Cox analysis. (C) Column chart. (D) Calibration curve of column chart model. CI, confidence interval; HCC, hepatocellular carcinoma; Mult_Cox, multivariate cox; pM, pathological metastasis; pN, pathological node; Pro, probability; pT, pathological tumor; RAC1 , Ras-related C3 botulinum toxin substrate 1; Uni_Cox, univariate cox.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques:

Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: The expression of RAC1 in HCC cell lines and the changes in the levels of RAC1 GTP in HCC cells before and after radiation. (A) Protein electrophoresis pattern. (B) Comparison of RAC1 expression levels. (C) Comparison of the changes in the levels of RAC1 GTP. *, P<0.05; **, P<0.01; *** P<0.001. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GTP, guanosine triphosphate; HCC, hepatocellular carcinoma; IR, ionizing radiation; RAC1 , Ras-related C3 botulinum toxin substrate 1.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques: Expressing, Protein Electrophoresis, Comparison

Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: The effect of RAC1 GTP on the p-IκBα/Bcl-xL signaling pathway in MHCC97-H HCC cells after radiation. (A) Protein electrophoresis map. (B) Comparison of p-IκBα expression levels. (C) Comparison of Bcl-xL expression levels. *, P<0.05. GTP, guanosine triphosphate; HCC, hepatocellular carcinoma; IR, ionizing radiation; RAC1, Ras-related C3 botulinum toxin substrate 1.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques: Protein Electrophoresis, Comparison, Expressing

Journal: Translational Cancer Research

Article Title: The effect and related mechanisms of RAC1 GTP on radiotherapy for hepatocellular carcinoma

doi: 10.21037/tcr-2025-987

Figure Lengend Snippet: The effect of RAC1 GTP on G2/M phase arrest in MHCC97-H HCC cells after radiation. (A) Cell cycle distribution map. (B) Comparison of different cell cycle proportions. (C) The proportion of different cell cycles. ****, P<0.0001. GTP, guanosine triphosphate; HCC, hepatocellular carcinoma; RAC1 , Ras-related C3 botulinum toxin substrate 1.

Article Snippet: The following immunochemical reagents were employed: rabbit monoclonal anti-RAC1 antibody (clone ARC03;cCat #CY-2103; CytoSkeleton, Inc., Denver, CO, USA), phospho-IκBα (Ser32) antibody [Cat #2859S; Cell Signaling Technology (CST), Inc., Danvers, MA, USA], IκBα antibody (Cat #4814S; CST), and rabbit polyclonal Bcl-xL antibody (clone ARC0183; Cat #A19703; ABclonal Technology Co., Ltd., Woburn, MA, USA).

Techniques: Comparison

Journal: Cell reports

Article Title: The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells

doi: 10.1016/j.celrep.2025.115401

Figure Lengend Snippet:

Article Snippet: Rabbit anti-Rac1 , Thermo Fisher Scientific , Cat# PA1-091; RRID: AB_2539856.

Techniques: Generated, Virus, Recombinant, SYBR Green Assay, Protease Inhibitor, Plasmid Preparation, In Situ, BIA-KA, Mutagenesis, Software